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RENAL BIOPSIES

Synonym(s):
Lab Section Category
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Test Description
  1. Label containers and complete Histo CPOE order.
  2. Renal biopsies are subjected to examination by light microscopy, electron microscopy and immunofluorescence. After the biopsy is done, samples must be divided carefully using a sharp blade to avoid crush artefact. A dissecting microscope is helpful in identifying tissue with glomeruli. The pieces of tissue are to be placed as quickly as possible into suitable containers with appropriate fixatives as indicated:
    (a) Light microscopy – modified Bouin's fluid (Dubossey fixative)
    (b) Electron microscopy – 2.5% glutaraldehyde in cacodylate buffer (the time for which the tissue is placed into fixative must be recorded)
    (c) Immunofluorescence – Fresh tissue is required. Specimen is wrapped in aluminum foil, placed in a cryotube and delivered to the lab on ice. Contact the laboratory (Tel: 6321 4008) for further information, if necessary.

Last Updated - 13 Jun 2025