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TISSUE PREPARATION FOR POLYMERASE CHAIN REACTION (PCR)/MOLECULAR

Synonym(s):

Lab Section Category

Description

​Tests perform by Histomolecular and molecular lab:

EGFR Sanger Sequencing, EGFR COBAS Real time PCR, KRAS/BRAF, NRAS, BRAF V600E, BRAF Sanger Sequencing, MSI PCR, C-KIT (Exon 9, 11), C-KIT (Exon 13, 17), PDGFRA, T & B Cell Clonality test, TB PCR, SSTP(Somatic Solid Tumour Panel)

  1. Refer to Immunohistochemistry for creating CPOE order.
  2. Requirements for external materials for Molecular test:
  3. For requests from external institutes, please raise a memo with the same information as above in point 1 and a copy of pathology report. Please indicate the billing location.
  4. Upon receiving or retrieving of sample, Histopathology lab takes 4-5 working days to submit the sample to the respective laboratory for testing. Please check with the respective laboratory (Molecular lab, Translational Pathology Centre, Cytogenetic lab and Central TB lab) for turnaround time of individual test performed by them.

Sample requirements:

Test Testing LaboratorySample requirementsTumour content
EGFR Sanger Sequencing SGH Molecular Lab 1 HE, 8 unstains at 8µm for resections, non coated slidesMinimum 30%
tumour content
1 HE, 12 unstains at 5µm for biopsies, non coated slides
EGFR Real Time PCR
(cobas)
SGH Molecular Lab 1 HE, 8 unstains at 8µm for resections, non coated slidesMinimum 10%
tumour content
1 HE, 12 unstains at 5µm for biopsies, non coated slides
KRAS/BRAFSGH Molecular LabRefer to requirement for EGFRMinimum 30%
tumour content
NRAS  SGH Molecular LabRefer to requirement for EGFRMinimum 30%
tumour content
BRAF V600ESGH Molecular LabRefer to requirement for EGFRMinimum 30%
tumour content
BRAF Sanger SequencingSGH Molecular LabRefer to requirement for EGFRMinimum 30%
tumour content
MSI PCR SGH Molecular Lab Please provide both tumour and normal tissueMinimum 30%
tumour content
1 HE, 8 unstains at 8µm for normal
1 HE, 8 unstains at 8µm for tumour
C-KIT (Exon 9,11)SGH Molecular LabRefer to requirement for EGFRMinimum 30%
tumour content
C-KIT (Exon 13, 17)SGH Molecular LabRefer to requirement for EGFRMinimum 30%
 tumour content
PDGFRASGH Molecular LabRefer to requirement for EGFRMinimum 30%
tumour content
T or B Cell
Clonality
SGH Molecular Lab 1 HE, 8 unstains at 8µm for resectionsMinimum 1%
lymphocyte content
1 HE, 20 unstains at 5µm for biopsies/ bone marrows
1p19q FISHCytogenetic Lab1HE, 4 unstains on coated slidesMinimum 100 nuclei
Lung Cancer FISH PanelCytogenetic Lab1HE, 8 unstains at 4µm on coated slidesMinimum 100 nuclei
Her-2 FISHCytogenetic Lab1HE, 3 unstains on coated slidesMinimum 100 nuclei
TB-PCR Central TB Lab 1 HE, 8 unstains at 8µm for resections, non coated slidesNot applicable 
1 HE, 15 unstains at 5µm for biopsies, non coated slides
SSTP Translational Pathology CentreRefer to requirement for EGFRMinimum 30%
tumour content

Contact laboratory (Tel: 6321 4008) for further information.

Indications

Specimen Required

Storage and Transport

Method

Test Results

Reference Interval / Value

Turnaround Time

Day(s) Test Set up

Remarks

Change History Notes

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