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PNEUMONIA MULTIPLEX PCR

Synonym(s):

​Mycoplasma pneumoniae, Chlamydophila pneumoniae, Chlamydia pneumoniae, Legionella pneumophila, Bordetella pertussis, Bordetella parapertussis, Atypical pneumonia     

Description

Indications

​For detection of Chlamydophila (formerly Chlamydia) pneumoniae DNA, Mycoplasma pneumoniae DNA, Legionella pneumophila DNA, Bordetella pertussis DNA and Bordetella parapertussis DNA which cause community-acquired atypical pneumonia.

Specimen Required

​(a) Sputum, nasopharyngeal aspirate or endo-tracheal tube aspirate: At least 1 mL of undiluted sample in a sterile leak-proof screw-capped container.

(b) Throat swab or tracheal swab: Use only Dacron-tipped swab. Send dry in sterile container or swab sheath.

Sputum is the preferred sample compared to throat swab.

Storage and Transport

​Refrigerate sample until transfer to laboratory. Do not freeze. Send sample at 2 - 8°C (with an ice-pack). Sample must reach the laboratory within 24 hours after collection.

Method

​Multiplex Real-time Polymerase Chain Reaction using Seegene Anyplex™ II RB5 Detection Kit

Test Results

​Detected, Not Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​1 - 4 days

Day(s) Test Set up

​Variable

Remarks

Change History Notes

  • 15 Nov 2013 06:30 PM

    Indications: Indications has been last updated on 15/11/13. Method: Method has been last updated on 15/11/13.

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