Synonym(s):
For detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV).
For the other respiratory pathogens (adenovirus, coronaviruses HKU1, NL63, 229E, OC43, human metapneumovirus, human rhinovirus/enterovirus, influenza A, influenza A/H1, influenza A/H3, influenza A/H1-2009, influenza B, parainfluenza viruses 1-4, respiratory syncytial virus, Bordetella parapertussis, Bordetella pertussis, Chlamydophila pneumoniae, Mycoplasma pneumonia) covered by this assay, our validation showed that both our current respiratory viruses multiplex PCR (in-house modified Seegene AnyplexTM II RV16) and our current Pneumonia multiplex PCR (Seegene AnyplexTM II RB5), consistently outperform this BioFire® FilmArray® Respiratory 2 plus Panel.
These specimens are to be sent in screw-capped sterile containers without adding viral transport media.
For urgent cases, please contact the laboratory (63266920/65767620). Urgent specimens must be hand-delivered to the Academia Building, Diagnostics Tower. Normal opening hours are from 8.00am to 5.10pm on Monday to Friday and 8.00am to 1.00pm on Saturdays.
All specimens must be transported to the laboratory promptly within a few hours of collection. If a delay in transport is anticipated, specimens should also be kept cold at 4°C, and thereafter delivered within 24 hours of collection.
Specimen can be transported at 18-25oC to the testing laboratory if the total storage and transport time at 18-25oC does not exceed one hour. Otherwise, specimen should be stored and transported cooled (2-8oC or with ice pack) and to reach the testing laboratory within 48 hours of collection.
Multiplex Real-time PCR
Routine: Next working day Urgent: 3 hours (require discussion with on-call microbiologist)
Mondays to Saturdays (Routine)
In addition to standard precautions, strict barrier precautions are required in the collection of respiratory samples from patients suspected to be infected with MERS coronavirus. This includes the use of personal protective equipment like gloves, gowns and apron, safety eyewear and powered air purifying respirators (PAPR) or N95 masks.
Note that urgent out-of-hours testing will only be done on inpatients in SGH. These must be ordered by an infectious diseases physician and discussed with Microbiologist-on-call for SGH Molecular Laboratory. Patient samples for urgent/stat testing must be from patients who meet MOH case definition of a suspect case.
Performance Characteristics:
Analytical Sensitivity (Manufacturer's claim):
Viruses
3000 IU/mL for Adenovirus (Species C, Serotype 2 - 100% confidence level)
330 copies/mL for Influenza A H1-2009 (100% confidence level)
21 copies/mL for Influenza A H3 (100% confidence level)
34 copies/mL for Influenza B (100% confidence level)
130 copies/mL for MERS-CoV (100% confidence level)
9 copies/mL for Respiratory Syncytial Virus (100% confidence level)
160 copies/mL for SARS-CoV-2 (100% confidence level)
Bacteria
41 CFU/mL for Bordetella parapertussis (IS1001 - 95% confidence level)
1000 CFU/mL for Bordetella pertussis (ptxP - 100% confidence level)
130 copies/mL for Chlamydia pneumoniae (100% confidence level)
460 copies/mL for Mycoplasma pneumoniae (100% confidence level)
Analytical Specificity (Manufacturer's claim):
No cross reactivity with Acinetobacter calcoaceticus, Bordetella avium, Bordetella bronchiseptica, Bordetella hinzii, Bordetella holmesii, Legionella bozemanii, Legionella dumoffii, Legionella feeleii, Legionella longbeacheae, Legionella micdadei, Legionella pneumophila, Chlamydia trachomatis, Corynebacterium diphtheriae, Enterobacter aerogenes, Escherichia coli, Haemophilus influenzae, Klebsiella oxytoca, Klebsiella pneumoniae, Lactobacillus acidophilus, Lactobacillus plantarum, Moraxella catarrhalis, Mycoplasma genitatlium, Mycoplasma hominis, Mycoplasma orale, Mycobacterium tuberculosis, Neisseria elongata, Neisseria gonorrhoeae, Neisseria meningitidis, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermis, Stenotrophomonas maltophilia, Streptococcus pneumoniae, Streptococcus agalactiae, Streptococcus pyogenes, Streptococcus salivarius, Ureaplasma urealyticum, BAT SARS-like Coronavirus (recombinant), BAT SARS-like Coronavirus HKU5 (recombinant), Severe Acute Respiratory Syndrome Coronavirus (SARS), Bocavirus, Cytomegalovirus (CMV), Epstein-Barr Virus (EBV), Herpes Simplex Virus 1, Herpes Simplex Virus 2, Human Herpes Virus 6, Human Parechovirus, Influenza C, Measles Virus, Mumps, Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus and Aspergillus flavus.
Assay limitations:
1. The Influenza A H1-2009 assay may react with H1 hemagglutinin gene sequences from viruses of swine origin. FilmArray RP2plus will report either Influenza A H1 or Influenza A H1-2009, depending on the strain and concentration in the sample. Swine origin Hsw1N1 (A/New Jersey/8/1976 ; ATCC VR-897) was detected as either Influenza A H1 or Influenza A H1-2009 at a concentration of 8.9E+06 CEID 50/mL.
2. At a sufficiently high concentration (eg.>4.5E+07 CFU/mL of Bordetella pertusis), cross reactivity may occur between Bordetella spp. and the Human Rhinovirus/Enterovirus assays. The following results may be seen:
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