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MENINGITIS MULTIPLEX PCR

Synonym(s):

Description

Indications

The FilmArray Meningitis/Encephalitis (ME) Panel is a qualitative multiplexed nuclei acid-based in vitro diagnostic test intended for use with FilmArray systems. The FilmArray ME Panel is capable of simultaneous detection and identification of multiple bacterial, viral, and yeast nucleic acids directly from cerebrospinal fluid (CSF) specimens obtained via lumbar puncture from individuals with signs and/or symptoms of meningitis and/or encephalitis. The following organisms are identified using the FilmArray ME Panel:

Bacteria:

  • Escherichia coli K1
  • Haemophilus influenzae
  • Listeria monocytogenes
  • Neisseria meningitidis (encapsulated)
  • Streptococcus agalactiae
  • Streptococcus pneumoniae

Viruses:

  • Cytomegalovirus
  • Enterovirus
  • Herpes simplex virus 1
  • Herpes simplex virus 2
  • Human herpesvirus 6
  • Human parechovirus
  • Varicella zoster virus

Yeast:

  • Cryptococcus neoformans/gattii

Specimen Required

At least 0.5 mL of CSF collected in a leak-proof sterile container. (Facemasks must be worn by all present during collection to prevent contamination of CSF sample.)

Storage and Transport

Specimens should be processed and tested with the FilmArray ME Panel as soon as possible, though they may be stored for up to one day at room temperature (approximately 23oC), or under refrigeration at 4oC for up to seven days.

Method

Multiplex real-time PCR

Test Results

Detected, Not detected or Inconclusive

Reference Interval / Value

Turnaround Time

24 hours

Day(s) Test Set up

Mondays to Sundays

Remarks

Change History Notes

Others

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