3 mL plain blood Paired acute and convalescent sera (3 – 6 weeks after onset of fever) should be sent. Acute phase sera are tested alone; a convalescent specimen is requested and when received will be run together with the acute phase sera.
Serum run at doubling dilutions from 1:64 to 1:1024. A fourfold rise in titre between acute and convalescent sera is considered proof of ongoing infection. A single titre of ≥ 1:1024 is considered a presumptive positive.
Range 1 – 4 days
Monday – Friday