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IDENTIFICATION OF M. BOVIS BCG AND NTM

Synonym(s):

Lab Section Category

Description

Indications

Specimen Required

​For mycobacteria isolated in CTBL or in other laboratories

Storage and Transport

​Fresh pure culture isolate on slant in sealed screw-capped plastic tube. Tube should be tightly closed, labeled with identifying lab number and placed in a biohazard-labelled ziplock bag. Pack, transport and notify CTBL (in advance) and MOH of the transfer, according to MOH-BATA requirements for M. tuberculosis complex.

Referred NTM for identification: Fresh pure culture isolate on slant in sealed screw-capped plastic tube. Tube should be tightly closed, labeled with identifying lab number and placed in a biohazard-labeled ziplock bag.

Regional laboratories must make arrangements with the lab for the MOH-Import Permit before sending specimens and packaging must conform to international standards (IATA).

The request form should state the name and address of referring laboratory, organism identification, clinical source, patient particulars with relevant clinical information, clinician in-charge and requester's contact number. The type of test request should be specified as Identification of BCG or NTM. Forms are to be placed in a separate sealed plastic bag in the outer package.

Method

​For BCG: HAIN MTBC
For NTM: line probe assays, MALDI-TOF and DNA probes

Test Results

​Mycobacteria species identified

Reference Interval / Value

Turnaround Time

​1 week to 2 months, depending on species

Day(s) Test Set up

​Monday - Friday

Remarks

​Referred M. tuberculosis complex isolates for identification of BCG from other laboratories- please contact CTBL before sending isolate.

Change History Notes

  • 29 Apr 2016 05:20 PM

    Amendment to Methods to include Hain MTBC and MALDI-TOF.

  • 12 Jan 2018 11:55 AM

    Remove HPLC in Methods

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