Skip Ribbon Commands
Skip to main content
Menu

HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) QUANTITATIVE PCR

Synonym(s):

​HIV-1 RNA Load

Description

Indications

​1) To obtain the baseline viral load in newly diagnosed cases of HIV-1 infection for prognostication.
2) To monitor disease progression in HIV-1 infected patients.
3) To monitor treatment response during the course of anti-retroviral therapy (ART).
Please note that this assay is NOT intended for use in the diagnosis of HIV-1 infection.
This test is able to detect HIV-1 group M subtypes and HIV-1 group O.      

Specimen Required

​At least 6 mL blood in sterile EDTA container (lavender top) (3 tubes).

Storage and Transport

​Transport sample at 2-25 °C to arrive in the laboratory preferably within 24 hours of collection and no later than 48 hours. Plasma may be transported at 2-8°C or frozen at -20 °C to -80 °C. Plasma specimens may be stored at room temperature (25-30°C) for up to 3 days, at 2-8 °C for up to 7 days or frozen at -20 °C to -80 °C for at least six weeks.

Method

​Real-time PCR (Roche COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Test, v.2.0)

Test Results

​Detected or Not Detected (quantity reported in copies/ml and IU/mL)
Linear Range : 20 to 1.00 X 107 copies/mL or 33 to 1.67 X 107 IU/mL

Reference Interval / Value

Turnaround Time

​1 - 8 days

Day(s) Test Set up

​Wednesday

Remarks

​The assay detects HIV-1 group M (subtypes A-H) and HIV-1 group O viruses.

Change History Notes

Others

TOP

What's New

Sort by :