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HUMAN IMMUNODEFICIENCY VIRUS (HIV)-1 RNA AND PROVIRAL DNA PCR (QUALITATIVE)

Synonym(s):

​HIV-1 Proviral DNA PCR

Description

Indications

​To determine the HIV infection status in
a. Infants born to HIV positive mothers
b. Individuals whose HIV screening test by EIA/MEIA is reactive, but whose status is indeterminate or inconclusive by Western blot
c. High-risk individuals with a negative serological screen and a history of recent exposure

This test is able to detect HIV-1 group M subtypes and HIV-1 group N & O.     

Specimen Required

​3 ml of whole blood collected in EDTA anticoagulant only

Storage and Transport

​Samples should be transported and stored at 2-8 oC (do not freeze) and should reach the testing laboratory within 48 hours of collection for whole blood processing (i.e. HIV-1 proviral DNA and HIV-1 RNA detection).

Method

​Real-time Qualitative Polymerase Chain Reaction (PCR), Roche COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test version 2.0.

Test Results

​Detected, Not Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​1 - 5 days

Day(s) Test Set up

​Tuesdays and Fridays (except public holidays)

Remarks

​Please note this test may not detect new variants of HIV-1. It also does not detect HIV-2 viruses.

Change History Notes

Others

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