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HEPATITIS C VIRUS (HCV) QUANTITATIVE PCR

Synonym(s):

​HCV RNA LOAD

Description

Indications

​For detection and quantitative measurement of hepatitis C viral (HCV) RNA in human serum or plasma. For monitoring response to treatment, where samples are obtained prior to and during treatment.

Specimen Required

​6 - 10 mL blood in sterile EDTA container or serum separation tubes. Alternatively, at least 3 mL plasma or serum (separated from EDTA-treated or whole blood within 6 hours of collection by centrifugation at 800-1600g for 20 minutes at room temperature) in a sterile screw-capped polypropylene tube.

Storage and Transport

​Refrigerate sample until transfer to laboratory. Do not freeze (except for plasma or serum). Send sample at 2 - 8°C (with an ice-pack). Sample must reach the laboratory within 24 hours after collection for plain blood and 48 hours for EDTA blood (preferably within 6 hours for all samples). Plasma may be stored at 2 - 8°C for up to 2 days or frozen at -70°C before despatch to the laboratory.

Method

​Real-time PCR (Roche COBAS Ampliprep/TaqMan HCV Quantitative Test, version 2.0).

Test Results

​Not detected or Detected (quantity reported in IU/mL)

Linear Range: 15 to at least 1.00E+08 IU/mL

Reference Interval / Value

Turnaround Time

​1 - 8 days    

Day(s) Test Set up

​Thursday

Remarks

Change History Notes

Others

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