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ENTEROVIRUS ISOLATION

Synonym(s):

​Enterovirus Culture, Coxsackie Virus Culture, Echovirus Culture, EV71 Isolation, Coxsackie Virus Isolation, Poliovirus Culture, Poliovirus Isolation

Lab Section Category

Description

Indications

Specimen Required

​1 – 2 mL CSF in sterile bottle for neurological diseases

Stool for all clinical presentations. Two stool samples collected 24 - 48 hours apart must be sent from all patients aged 15 years and below with a diagnosis of AFP (acute flaccid paralysis) for poliomyelitis surveillance.

Throat swab/nasopharyngeal aspirate within the first 3 – 4 days of onset for all clinical presentations

Throat swab, nasopharyngeal aspirate or bronchoalveolar lavage for respiratory infections

Pericardial fluid for pericarditis

Vesicular fluid for vesicular eruptions

Send samples (except CSF, stool, pericardial fluid & vesicular fluid) in Virus Transport Medium.
(See also Virology Section, General Guidelines for Virus Isolation & Collection Methods for Virus Isolation by Sample Type)

Storage and Transport

​Refrigerate samples until transport to laboratory. Do not freeze.

Send samples on ice pack to laboratory.

Method

​Tissue culture

Test Results

​Reported as Positive or Negative

Reference Interval / Value

Turnaround Time

​Positive preliminary report : 3 days – 3 weeks

Final report with enterovirus typing results : 2 - 6 weeks

Negative report : 3 weeks

Day(s) Test Set up

​Monday – Saturday

Remarks

Change History Notes

  • 29 Dec 2017 02:40 PM

    Changed turn around time (TAT) for positive preliminary report to 3 days – 3 weeks.

  • 02 May 2018 04:50 PM

    The timing of collecting two stool samples from AFP patient changed.

Others

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