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CYTOMEGALOVIRUS (CMV) QUANTITATIVE PCR (EDTA BLOOD)

Synonym(s):

Description

Indications

​For detection and quantification of CMV DNA in patient’s plasma, as an adjunct for the diagnosis or prediction of CMV disease, i.e. from reactivation of latent disease in immunocompromised patients or in primary infection, or for monitoring treatment progress in CMV disease.

Specimen Required

​5 mL of blood in EDTA (lavender top) tube. Alternatively, at least 3 mL plasma (separated from EDTA-treated or whole blood within 6 hours of collection by centrifugation at 800-1600g for 20 minutes at room temperature) in a sterile screw-capped polypropylene tube.

Please note that sample types other than blood in EDTA or plasma have not been validated on this commercial system. Quantitation of CMV load in other sample types e.g. cerebrospinal fluid, aqueous/vitreous fluid, will be performed on our in-house quantitative assay. See CMV Quantitative PCR (Non-Blood).

Storage and Transport

​Refrigerate sample at 2-8°C until transfer to laboratory. Do not freeze. Send sample at 2 - 8°C. Plasma may be transported at 2-8°C or frozen at ≤-20°C. Sample should reach the laboratory as soon as possible preferably within 6 hours after collection.

Method

​Roche COBAS® AmpliPrep/COBAS® TaqMan CMV test

Test Results

​Detected (quantity reported in copies/mL as well as IU/mL), Undetectable or Inconclusive.

Linear Range: 150 - 10,000,000 copies/mL (2.18 - 7.00 log)
                          137 - 9,100,000 IU/mL           (2.14 - 6.96 log)

Reference Interval / Value

Turnaround Time

​4 days

Day(s) Test Set up

​Monday to Friday (except public holidays)

Remarks

Change History Notes

Others

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