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CHROMOSOME ANALYSIS OF BONE MARROW/BONE CORE/HEMATOLOGIC DISORDERS BLOOD/EFFUSION FLUID

Synonym(s):

​KARYOTYPE BONE MARROW/BONE CORE/HEMATOLOGIC DISORDERS BLOOD/EFFUSION FLUID

Description

Indications

​- Acute Leukaemia
- Lymphoma
- Myelodysplastic Syndromes
- Chronic Myeloid Leukaemia
- Other Lymphoproliferative Disorders

Specimen Required

​Bone marrow (1.5ml - 2ml) in sterile sodium/lithium heparin. A blood specimen (5 - 10ml) in sodium/lithium heparin will yield results if blast cells are present in large numbers (preferably ≥25%). Blood can also be used for investigation of Lymphoproliferative Disorders (LPD), in particular, Chronic Lymphocytic Leukaemia (CLL). Do not use EDTA. The specimen should be taken from the first or second aspirate and it should not be haemodiluted. If the first aspirate of marrow is inadequate, it may be helpful to rotate the needle 1800 to minimise blood contamination before re-taking the marrow sample. Effusion Fluid can be taken in a sodium/lithium vacutainer.

Storage and Transport

Method

​24-hour and 48-hour or 72-hour cultures. A stimulated culture is set up for LPD and multiple myeloma (MM).

Test Results

​Normal or Abnormal. If Abnormal, the karyotypic abnormality is described in accordance to the International System for Human Cytogenomic Nomenclature (ISCN, 2016).

Reference Interval / Value

Turnaround Time

​14 ~ 21 days

Day(s) Test Set up

​Monday – Saturday (office hours)

Remarks

Indicate clearly the clinical diagnosis and white cell count (if any) on the cytogenetics request form or CPOE. This information is essential when initiating the cultures for cytogenetic analysis. Do not despatch a specimen near a weekend or a public holiday to avoid unnecessary delays.

Change History Notes

Others

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