The T & B Cell Clonality is intended for PCR-based detection of clonal IGH chain, IGK light chain, TCRB chain and TCRG chain gene rearrangements in patients with suspect lymphoproliferations.
Send formalin fixed paraffin embedded (FFPE) tissue block to the Histopathology Section, Department of Pathology, SGH. If sending slide sections, send at least 8 sections of 8 micrometres thickness, or at least 20 sections of 5 micrometres thickness. One H&E slide, 4 micrometre thick, with a coverslip as a reference slide for the unstained slides, must also be included.
PCR (using BIOMED-2 primer sets) and fragment analysis
Clonality detected, Polyclonality detected, No clonality detected, Inconclusive
Monday and Wednesday
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