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25 HYDROXYVITAMIN D TOTAL, SERUM

Synonym(s):
Lab Section Category
View write-up
Specimen Required

​5 mL plain blood

Indications

For diagnosis of Vitamin D deficiency and monitoring of response to Vitamin D therapy

Method

​Liquid Chromatography - Tandem Mass Spectrometry

(LC-MS/MS)

Test Results A profile comprising of 25 Hydroxyvitamin D2, D2 and Total will be reported:

(1) 25 Hydroxyvitamin D3

(2) 25 Hydroxyvitamin D2

(3) 25 Hydroxyvitamin D, total (D3+D2)

Reference Interval / Value

​Deficient < 20 ng/mL

Vitamin D supplementation to a level above 30 ng/mL may be appropriate for at-risk individuals on anti-osteoporosis therapy.

Levels above 50 ng/mL may be associated with potential adverse effects.

Turnaround Time

​Mean = 5 days, Range = Up to 7 days

Day(s) Test Set up

​Monday - Friday

Change History Notes

  • 02 Nov 2020 09:00 AM

    ​New test available w.e.f 02 Nov 2020

Back: Pathology Laboratory Services

Last Updated - 27 Oct 2025