For diagnosis of Vitamin D deficiency and monitoring of response to Vitamin D therapy
5 mL plain blood
Liquid Chromatography - Tandem Mass Spectrometry
(1) 25 Hydroxyvitamin D3
(2) 25 Hydroxyvitamin D2
(3) 25 Hydroxyvitamin D, total (D3+D2)
Deficient < 20 ng/mL
Vitamin D supplementation to a level above 30 ng/mL may be appropriate for at-risk individuals on anti-osteoporosis therapy.
Levels above 50 ng/mL may be associated with potential adverse effects.
Mean = 2 days, Range = Up to 5 days
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