HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) QUANTITATIVE PCR

3-5 mL of blood in sterile EDTA (lavender top) tube. Alternatively, at least 3 mL plasma (separated from EDTA-treated or whole blood within 6 hours of collection by centrifugation at 3000 rpm for 10 minutes at room temperature) in a sterile screw-capped polypropylene tube.  

Please note that sample types other than blood in EDTA or plasma have not been validated on this commercial system.

Refrigerate sample at 2-8°C until transfer to laboratory. Do not freeze.  Plasma may be transported at 2-8°C or frozen at ≤-20°C. Sample should reach the laboratory as soon as possible, preferably within 6 hours after collection.

​Real-time Quantitative Reverse Transcription PCR (Roche COBAS 6800 HIV-1 Test)

​Detected or Not Detected (quantity reported in copies/ml and IU/mL)
Linear Range : 20 to 1.00 X 10⁷ copies/mL or 33 to 1.67 X 10⁷ IU/mL

​1 - 8 days

​Wednesday and Friday

The assay detects HIV-1 group M (subtypes A-D, F-H, CRF01_AE, CRF02_AG), group N and group O viruses. Please note this assay does not detect nor quantitate HIV-2 virus.