CINTEC PLUS CYTOLOGY

​CINtec® PLUS Cytology Kit is an immunocytochemistry assay for simultaneous qualitative detection of p16^INK4a and Ki-67 proteins in cervical cytology preparations.

​​1. Cytologic sample collected in PreservCyt® Solution (Hologic Inc.) - see site specific instruction on specimen collection and handling of (A) Gynaecological Cytology for details. (Do not use lubricant as it will affect the quality of the smear prepared).

A cytology gynaecological CPOE request that is properly filled up - see the CPOE request for details. 

OR

2. Archived specimen from Molecular Laboratory that is no more than 6 weeks from collection date may be used.

Alert Cytology lab (6576 7587) of the additional request. Put up a new cytology gynaecological CPOE request that is properly filled up and send the CPOE label to Cytology Lab.

​The test indicates oncologically transforming HPV infections and is indicated to be used as an aid in the identification of women with high-grade cervical intraepithelial lesions in a screening population, and in the sub-groups of patients with a Pap cytology result of ASC-US (atypical squamous cells of undetermined significance) or LSIL (low-grade squamous intraepithelial lesion), or in patient with positive high-risk HPV test result.

​In the Thinprep® 2000 processor (Hologic Inc.), the specimen undergoes dispersion and cells are collected on a Thinprep® Pap Test filter before a monolayer of cells is tranferred onto a glass slide. 

The glass slide is stained with the CINtec® PLUS Cytology Kit is optimized for use on VENTANA BenchMark GX, XT and ULTRA autostainers. The p16^INK4a and Ki-67 antigens in the specimens are detected using a ready-to-use cocktail of primary monoclonal antibodies which contains a monoclonal mouse antibody (clone E6H4™) directed against human p16^INK4a protein  and a primary recombinant rabbit antibody (clone 274-11 AC3) against human Ki-67 protein .

The stained slides are evaluated by light microscopy inspection and are to be interpreted in conjunction with patient’s clinical history and additional diagnostic tests that have been performed.

7 working days from date of run - every Wednesday and Friday

Wednesday and Friday (Lab to receive specimen before noon)