Somatic Solid Tumour Comprehensive Panel (SSTP Comprehensive)

Unstained formalin fixed paraffin embedded (FFPE) tissue sections on uncoated slides (8 sections of 8 µm thickness for large resection / excision specimens or 15 sections of 5 µm thickness for small biopsy specimens) together with 1 H&E stained slide is required for this assay. Sections from cell block specimens are also acceptable (15 sections of 5 µm thickness). 

The tissue/cell block sections should ideally contain at least 20% of tumour cells. Specimens with lower tumour cellularity can still be accepted for testing but the sensitivity for detection of molecular alterations may be reduced. 

Paraffin-embedded tissue blocks are also acceptable. 

DNA/RNA is accepted only if FFPE tissue is not available. The laboratory only accepts DNA/RNA that is extracted or isolated in a CAP-accredited / CLIA-certified or equivalent laboratory. Please call the lab for additional details.

The SSTP Comprehensive assay is able to characterise somatic molecular alterations in tumours for diagnostic, prognostic or predictive purposes. It is indicated in patients with tumours who require testing for somatic molecular alterations in the genes covered by the assay, as deemed necessary by the managing physician.

Click here to view more details on SSTP Comprehensive assay including the full list of genes covered.

Specimens should be sent to 20 College Road, Academia, Diagnostic Tower Level 10, Histopathology Lab, Department of Anatomical Pathology, Singapore General Hospital, Singapore 169856.

Any queries can be directed to the Anatomical Molecular Laboratory (ATOM) at (65) 6576 7191 or Histopathology Laboratory at (65) 6321 4956.

Targeted panel NGS assay that simultaneously analyses both DNA and RNA in one integrated workflow. The panel detects multiple variant types and biomarkers across 523 cancer-relevant genes from DNA and 55 genes from RNA. Library preparation is based on hybrid-capture chemistry with unique molecular identifiers (UMIs) added for error correction to achieve high analytical specificity.

Single nucleotide variants, small insertions / deletions, copy number variants, microsatellite instability (MSI) status and tumour mutational burden (TMB).

Genomic Instability Score (GIS) calculation, powered by Myriad Genetics, if the homologous recombination repair deficiency (HRD) workflow is added.

Gene fusions and RNA exon splice variants (MET exon 14 skipping, EGFR vIII and AR-V7).

15 to 20 working days from day of test set-up

Every Thursday.