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PDGFRA Mutation Analysis (Exons 12, 14 and 18)

Synonym(s):

Description

Indications

​Screening of GIST tumor samples for mutations in the PDGFRA gene may be helpful in the diagnosis, prognostication and management of patients with GIST. These assays detect mutations from formalin-fixed paraffin-embedded GIST samples.

Specimen Required

​Paraffin embedded tissue block sent to the Histopathology Section.


Alternatively, unstained slides can be sent to the Histopathology Section. At least 8 sections of 8 µm thickness, or 12 sections of 5 µm thickness for smaller biopsies should be sent together with one H&E slide, 4 µm thick with a coverslip as a reference slide. Tumour content must be indicated and must be at least 30%.

Storage and Transport

Method

​Polymerase Chain Reaction followed by direct Sanger Sequencing (In-house test)

Test Results

​Detected, Not Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​5-10 days

Day(s) Test Set up

​Variable

Remarks

Change History Notes

  • 05 Dec 2012 04:40 PM

    New test available w.e.f. 10 December 2012

  • 04 Dec 2015 12:40 PM

    Turnaround Time: Turnaround Time has been updated on 04/12/15.

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