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KRAS/BRAF MUTATION ANALYSIS

Synonym(s):

Description

Indications

​This assay detects 18 mutations in codons 12, 13, 61, 117 and 146 of the KRAS gene and V600E in exon 15 of the BRAF gene. The presence of these mutations correlates with a lack of response to certain EGFR inhibitor cancer therapies in metastatic colorectal cancer patients.

Specimen Required

​Paraffin embedded tissue block sent to the Histopathology Section.
Alternatively, unstained slides can be sent to the Histopathology Section.
Note :
- At least 8 sections of 8 mm thickness, or 12 sections of 5 mm thickness for smaller biopsies should be sent together with one H&E slide, 4 mm thick with a coverslip as a reference slide.
- Tumour content must be indicated and must be at least 30%.

Storage and Transport

Method

​EntroGen KRAS/BRAF Mutation Analysis Kit (v1.2), using allele-specific real-time PCR.

Test Results

​Detected, Not Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​10 days   

Day(s) Test Set up

​Variable

Remarks

Change History Notes

  • 15 Jan 2013 08:40 AM

    Turnaround time updated on 15.1.2013.

  • 16 Jan 2015 04:50 PM

    Title: Title has been last updated on 16/01/15. Indications: Indications has been last updated on 16/01/15. Day(s) Test Set-Up: Day(s) Test Set-Up has been last updated on 16/01/15.

  • 19 Jan 2015 08:35 AM

    Method: Method has been last updated on 19/01/15.

  • 16 Jan 2017 02:45 PM

    Turnaround Time: Last updated on 16/01/17. Day(s) Test Set up: Last updated on 16/01/17.

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