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INTERPHASE FLUORESENCE IN SITU HYBRIDIZATION PANEL TEST FOR GLIOMA

Synonym(s):

​GLIOMA FISH PANEL

Description

Indications

​This FISH test uses the LSI 1p36 / LSI 1q25 & LSI 19q13 / LSI 19p13 dual colour probe sets to detect chromosomal deletions involving the 1p36 and 19q13 regions.

Specimen Required

​Freshly-cut tissue sections.

Storage and Transport

​The FISH test is optimal with freshly-cut tissue samples. Tissue sections should preferably be between 4 - 6mm in thickness on coated/positively-charged slides. The optimal fixation time in formalin should be between 6 - 72 hours. An accompanying Hematoxylin and Eosin (H&E) stained slide with the tumour region marked out by a pathologist should be submitted together with at least 6 unstained sections.

Method

​Fluorescence In Situ Hybridization using direct-labelled FISH DNA probes are hybridized to target loci and analysed under fluorescence microscopy.

Test Results

​Findings are reported in accordance to the International System for Human Cytogenomic Nomenclature (ISCN, 2016).

Reference Interval / Value

Turnaround Time

​3 ~ 10 days

Day(s) Test Set up

​Monday – Saturday (office hours)

Remarks

Change History Notes

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