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IDENTIFICATION OF MYCOBACTERIA

Synonym(s):

​Mycobacterial Identification

Lab Section Category

Description

Indications

Specimen Required

​Referred cultured acid-fast positive isolate from other laboratories

Storage and Transport

​Fresh pure culture of isolate on slant in sealed, screw-capped plastic tube. Local laboratories may submit positive MGIT tubes or Myco-F lytic cultures, but these must be packed and labelled properly.

All containers should be tightly closed, labeled with identifying lab number and placed in a biohazard-labelled ziplock bag. Pack and transport according to MOH-BATA requirements.

Request form (stating name and address of referring laboratory, source of mycobacterial isolate, patient particulars with relevant clinical information, clinician in-charge and requester's contact number) is to be placed in separate sealed plastic bag outside the ziplock bag. The type of test should be specified as Mycobacterial Identification. For regional labs, packaging must conform to IATA standards.

Method

​AFB smear, culture, DNA probes, line probe assays and MALDI-TOF.

Test Results

​Species identified reported

Reference Interval / Value

Turnaround Time

​2 - 4 weeks depending on the nature of the organism

Day(s) Test Set up

​Monday - Friday

Remarks

​Please contact CTBL at tel: 6576 7655/7656 before sending isolate

Change History Notes

  • 16 Oct 2015 06:15 PM

    Amend CTBL tel no. from 6222 1391 to 6576 7655/7656

  • 29 Apr 2016 05:25 PM

    Amend Storage and Transport.

  • 12 Jan 2018 12:00 PM

    Remove HPLC in methods

Others

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