HIV-1 RNA Load
1) To obtain the baseline viral load in newly diagnosed cases of HIV-1 infection for prognostication. 2) To monitor disease progression in HIV-1 infected patients. 3) To monitor treatment response during the course of anti-retroviral therapy (ART). Please note that this assay is NOT intended for use in the diagnosis of HIV-1 infection. This test is able to detect HIV-1 group M subtypes and HIV-1 group O.
At least 6 mL blood in sterile EDTA container (lavender top) (3 tubes).
Transport sample at 2-25 °C to arrive in the laboratory preferably within 24 hours of collection and no later than 48 hours. Plasma may be transported at 2-8°C or frozen at -20 °C to -80 °C. Plasma specimens may be stored at room temperature (25-30°C) for up to 3 days, at 2-8 °C for up to 7 days or frozen at -20 °C to -80 °C for at least six weeks.
Real-time PCR (Roche COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Test, v.2.0)
Detected or Not Detected (quantity reported in copies/ml and IU/mL) Linear Range : 20 to 1.00 X 107 copies/mL or 33 to 1.67 X 107 IU/mL
1 - 8 days
The assay detects HIV-1 group M (subtypes A-H) and HIV-1 group O viruses.
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