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HERPES SIMPLEX VIRUS (HSV) PCR (QUALITATIVE)

Synonym(s):

​HSV PCR

Description

Indications

​For detection and typing of herpes simplex virus (HSV) in patients suspected of having HSV encephalitis, uveitis and HSV infections in other sites.

Specimen Required

​(a) CSF: At least 0.5 mL of undiluted sample in a sterile and leak-proof screw-capped container without any additives.

(b) Swab for vesicles: Use Dacron-tipped swabs. Sample base of lesions vigorously and send dry in swab sheath.

(c) Blood: 1-3 mL of EDTA whole blood only for suspected neonatal HSV infection or HSV hepatitis.

Storage and Transport

​Refrigerate sample until transfer to laboratory. Do not freeze. Send sample at 2 - 8°C (with an ice-pack). Sample must reach the laboratory within 24 hours after collection.

Method

​In-house real-time multiplex Polymerase Chain Reaction (PCR).

Test Results

​Detected, Not Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​1 - 4 days; next working day for CSF samples

Day(s) Test Set up

​Tuesday and Friday

Remarks

Change History Notes

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