Skip Ribbon Commands
Skip to main content
Menu

HEPATITIS C VIRUS PCR

Synonym(s):

Description

Indications

1.Diagnostic Purposes

  1. For detection of Hepatitis C virus (HCV) viraemia prior to sero-conversion in cases of high risk exposures.
  2. For confirmation of equivocal HCV serology results.
  3. To detect HCV viraemia in immunosuppressed individuals.
  4. For detection of HCV viraemia (which may be fluctuating) in anti-HCV antibody-positive patients or to diagnose chronic infection.

2.Monitoring Purposes

a. For monitoring response to treatment, where samples are obtained prior to, during and/or after treatment.

Specimen Required

6 - 10 mL blood in sterile EDTA container or serum separation tubes. Alternatively, at least 3 mL plasma or serum (separated from EDTA-treated or whole blood within 6 hours of collection by centrifugation at 800-1600g for 20 minutes at room temperature) in a sterile screw-capped polypropylene tube.

Storage and Transport

Refrigerate sample until transfer to laboratory. Do not freeze (except for plasma or serum). Sample must reach the laboratory within 24 hours after collection for plain blood and 48 hours for EDTA blood (preferably within 6 hours for all samples). Plasma may be stored at 2 - 8°C for up to 2 days or frozen at -70°C before despatch to the laboratory.

Method

​Real-time Quantitative Polymerase Chain Reaction (qPCR) (Roche COBAS 6800 HCV test)

Test Results

​Not detected or Detected (quantity reported in IU/mL)

Linear Range: 15 to 1.00E+08IU/mL

Reference Interval / Value

Turnaround Time

​1 - 8 days

Day(s) Test Set up

Tuesday and Thursday

Remarks

Change History Notes

Others

TOP

What's New

Sort by :