CMV IgG Antibody, CMV IgG, CMV IgM Antibody, CMV IgM, CMV CFT (blood), CMV Total Antibody (blood)
Cytomegalovirus IgM Antibody (anti-CMV IgM) Cytomegalovirus IgG Antibody (anti-CMV IgG) Cytomegalovirus CF Total Antibody (anti-CMV total)
3 – 5 mL plain blood. (a) To diagnose acute infection, send acute sample for CMV IgM Antibody and second serum taken 14 days later for CMV CF Total Antibody on the pair. (b) To diagnose previous infection, send single sample for CMV IgG Antibody.
Refrigerate samples if delay in transporting to laboratory is anticipated. Do not freeze.
Anti-CMV IgM : Enzyme Immunoassay Anti-CMV IgG : Chemiluminescent Microplate Immunoassay Anti-CMV total : Complement Fixation Test (CFT)
Acute infection: IgM Ab positive and/or paired sera showing ≥ 4 fold rise of CF Ab titres. Single sample having CF Ab titre ≥ 128 is suggestive of a recent infection. Confirm by virus isolation. Previous (latent) infection: CMV IgG present.
Anti-CMV IgM : <1 – 7 days (Negative report) <1 – 14 days (Positive / Equivocal report) Anti-CMV IgG : <1 – 4 days Anti-CMV total : 1 – 6 days
Anti-CMV IgM : Monday
Anti-CMV IgG : Tuesday and Friday
Anti-CMV total : Tuesday and Thursday