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CLOSTRIDIOIDES (FORMERLY CLOSTRIDIUM) DIFFICILE PCR (QUALITATIVE)

Synonym(s):

Description

Indications

​a) Direct testing for suspected C. difficile infection.
b) Confirmation of discordant enzyme immunoassay results, i.e. when stool samples tested positive for Glutamate Dehydrogenase (GDH) but negative for toxin production.

Specimen Required

​Stool (unformed)

Storage and Transport

​Refrigerate sample until transfer to laboratory. Do not freeze. Send sample at 2 - 8°C (with an ice-pack). Sample should reach the laboratory as soon as possible, preferably within 24 hours after collection.

Method

​Commercial real-time PCR - Cepheid Xpert C. difficile

Test Results

​Detected, Not Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​1 working day

Day(s) Test Set up

​Monday to Saturday

Remarks

​This PCR assay detects Toxin B gene only for all toxigenic C.difficile. This test can also presumptively identify the hypervirulent C. difficile 027/BI/NAP1 epidemic strain, but may not detect certain rare strains of C.difficile.

Change History Notes

Others

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