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BRAF MUTATION ANALYSIS BY SEQUENCING FOR MELANOMA

Synonym(s):

Description

Indications

​The test is used for detection of BRAF mutations in codons 594 - 601 (c.1780-c.1803) in exon 15 of the BRAF gene from Melanoma FFPE samples.
1. As a prognostic and highly predictive factor of increased response to therapy with BRAF inhibitors.
2. Assessment of clonal identity in multifocal melanoma lesions.

Specimen Required

​Paraffin embedded tissue block sent to the Histopathology Section.
Alternatively, unstained slides can be sent to the Histopathology Section. At least 8 sections of 8 micrometers thickness, or 12 sections of 5 micrometers thickness for smaller biopsies should be sent together with one H&E slide, 4 micrometers thick with a coverslip as a reference slide. Tumour content must be indicated and must be at least 30%. 

Storage and Transport

Method

​Direct PCR sequencing (in-house test)

Test Results

​Specific mutations detected, No mutations Detected or Inconclusive

Reference Interval / Value

Turnaround Time

​5 - 10 days

Day(s) Test Set up

​Variable

Remarks

Change History Notes

Others

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