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HEPATITIS B VIRUS (HBV) QUANTITATIVE PCR

Synonym(s):

​HBV DNA LOAD

Description

Indications

​For detection and quantitative measurement of hepatitis B viral DNA in serum or plasma. For prognostication, guiding treatment decisions and monitoring response to treatment.

Specimen Required

​3 – 5 mL blood in sterile EDTA container or serum separation tube. Alternatively, at least 1.5 mL plasma or serum (separated within 1 day of collection by centrifugation at 800-1600 X g for 20 minutes at room temperature) in a sterile screw-capped polypropylene tube.

Storage and Transport

​Refrigerate sample until transfer to laboratory. Do not freeze (except plasma or serum). Sample should reach the laboratory as soon as possible, preferably within 24 hours after collection. Plasma may be stored at 2 - 8°C for up to 2 days or frozen at -70°C before despatch to the laboratory.

Method

​Real-Time PCR (Roche COBAS Ampliprep/TaqMan) version 2.0

Test Results

​Detected or Not Detected (quantity reported in copies/mL and/or IU/mL)

Linear Range: 20 to 1.7 X 108 IU/mL (116 to 9.89 X108 copies/mL)

Lowest detection limit : 9 IU/mL

Reference Interval / Value

Turnaround Time

​1 - 5 days

Day(s) Test Set up

​Monday and Thursday

Remarks

Change History Notes

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